Biomedical Engineering Reference
In-Depth Information
FIGURE 8.5
(a, b) Fluorescence confocal microscopy of hierarchical cocultured cell
beads. (a) Collagen gel beads encapsulating HepG2 cells (red) and seeded with 3T3
cells (green) after 17 h of incubation.
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(b) Self-assembling peptide beads containing
endothelial cells (red) and 3T3 cells (green) after 2 days of culture.
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(c) Fluorescent
imageofacollagengelrodseededwithHUVECcellsshowingtherodcoveredwitha
confluent cell layer.
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(d) Image of the modular construct, including the microporous
body.
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(e) Schematic suggesting assembly of cell-laden hydrogel blocks in oil. Mechanical
agitation applied using a pipette tip and secondary cross-linking form diverse shapes of gel
assemblies: random, branched, linear, and offset.
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(f, g) Images of the lock-and-key-shaped
gel assembly containing three rods per cross. Cross-shaped gel and rod-shaped gel
encapsulates red-labeled and green-labeled 3T3 cells, respectively. Scale bars,
200
m
m.
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(h) Schematic diagrams of the assembly of cell-laden hydrogel blocks to
form lined-shaped cell structures in a microfluidic device.
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(i) Image of the line-shaped
cell structure consisting of three differently labeled cells.
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(j)Scanningelectronmicro-
scope image of the microtrain.
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(k) Guided movement of microtrain along a reentrant
rail.
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(l) Image of the Eiffel Tower assembly produced by the guided assembly method.
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(m) Image of the rail-guided assembly including two different types of living cells. Green
and red indicate HeLa and HEK293 cells, respectively.
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(c, d) Copyright 2006 National
Academy of Science, (e-g) copyright 2008 National Academy of Science, (h, i) copyright
2008 Royal Society of Chemistry, (j-m) copyright 2008 Nature Publishing Group.
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