Biology Reference
In-Depth Information
Table 3.1 Expression profiling experiments that compare neurons of differing regenerative potential
Number of
genes on
chip
Number of
differentially
expressed genes
Regenerating
cell/injury
Control cell/
injury
Time
postinjury
CNS development
Profiling method
Species
Threshold
Arlotta et al. (2005)
Microarray
(Affymetrix
430A)
14,000
Mouse CST (FACS
purified)
Na
Uninjured—
2-fold
change
1037
E18 to P14
>
Wang et al. (2007)
Microarray
(Affymetrix
U34ABC)
13,000
Rat
RGC
(immunopan)
Na
Uninjured—
E17 to P21
3-fold
change
2226
>
PNS regeneration
Fan, Mi, Yew, and
Chan (2001)
Oligonucleotide
array
580
Mouse Ventral spinal
cord/sciatic
nerve crush
motor cortex/
spinal
transection
3 days
Qualitative
dot intensity
80
Costigan et al. (2002)
Microarray
(Affymetrix
U34A)
8799
Rat
DRG/sciatic
nerve transection
Uninjured
DRG
3 days
1.5-fold
change
280
>
Xiao et al. (2002)
cDNA array
7523
Rat
DRG/sciatic
nerve transection
Uninjured
DRG
2, 7, 14,
28 days
>
2-fold
173 (152 known)
Schmitt et al. (2003)
Differential
display PCR
NA
Mouse
Facial motor
neurons
Red nuc./
spinal cord
hemisection
7 days
Qualitative
band
intensity
135 (69 known)
Tanabe, Bonilla,
Winkles, and
Strittmatter (2003)
Microarray
(Affymetrix
U74ABC)
8000
Mouse DRG/sciatic
nerve transection
Uninjured
DRG
7 days
5-fold
36
>
