Biology Reference
In-Depth Information
Table 9.4
Treatment methods based on the use of
Lactobacillus
sp. for fi sh waste remediation (parameters, quality control and results)
No.
Kind of
waste
Treatment
Parameters
Methodology
Quality control methods
Results
Reffer-
ences
1.
Sardine fi sh
waste
Fermenta-
tion
pH, dry matter,
fat content, total
nitrogen, non-
protein nitrogen
Chopped, mixed with 15%
molasses, inoculated with
Lactobacillus plantarum, and
incubated at 22°C for 20 days.
Incorporation with bran and
barley
1. pH: pH-meter
2. Dry meter: oven drying at 105°C
3. Fat content: Soxhlet method
using hexane as solvent
4. Total nitogen: Kjeldhal method
5. Non-protein nitrogen: Kjeldhal
method
1. pH decrease in the
fermenting product
(then remained constant
at 4.2-4.5)
2 Slight total nitrogen
decrease (5.3-5.7%)
3. Non-protein nitrogen
increase (220-262%)
Ham-
moumi et
al., 1998
Experiment 1:
Mixture of 1.5% propionic/ for-
mic acid (1:1) and crab-process-
ing waste ensiled with wheat
straw, sugarcane molasses, and
water (32:32:16:20, wet basis)
with or without 0.1% microbial
inoculant (
Streptococcus faecium
and
Lactobacilus
plantarum
)
Experiment 2:
Application of either 0.2%
sodium hypochlorite (NaOCl)
or 0.4% hydrogen peroxide
(H
2
O
2
) to crab waste. Ensiling
of the mixture as described in
Treatment A
Experiment 3:
Treatment of crab-waste with
1% sodium nitrate (NaNO
2
)
Experiment 1:
1. Prevention of waste
degradation for up to
14days
2. 16% pH and 48.9%
watersoluble carbohy-
drates (WSC) decrease
3. 252.1% lactic acid
increase
Experiment 2:
1. Prevention of deteriora-
tion for up to 7 days
2. 246.5% trimethylamine
(TMA) concentration
increase for NaOCl
treated waste
3. 1.87% TMA concentra-
tion increase when
2.
Crab-
processing
waste
Fermenta-
tion
Trimethlylamine
(TMA), nitro-
gen, dry matter
(DM), ash, pH,
lactic acid, water
soluble carbohy-
drates (WSC)
1. Trimethylamine (TMA):
colorimetric procedure (Dyer,
1959) after extraction with 7.5%
trichloroacetic acid
2. Nitrogen: Kjeldahl method
(AOAC, 1984)
3. Dry matter (DM): drying in a
forced draft oven at 60°C for 48h
4. Ash: AOAC (1984)
5. pH: elecrometrically
6. Latic acid: method of
Banker and Summerson (1941)
(modifi ed by Pennington and
Sutherland,1956)
7. Water soluble carbohydrates
(WSC): of Dubois et al. (1956)
(adapted to corn plants by
Johnson et al., 1966)
Ayangbile
et al., 1997
