Molecular Detection of Seafood-Borne Human Pathogenic Bacteria - Aquaculture Microbiology and Biotechnology - page 124

which yielded only two profi les. RAPD analysis of the group II strains
was not consistently reproducible with primers OPJ-6 and OPJ-13 ( Table
5.2 ) . Primer OPJ-13 did however yield 28 profi les.

ABBREVIATIONS
APS Alkaline Peptone Salt

APW Alkaline Peptone Water

CFU Colony Forming Units

CTX Cholera toxin

ELISA Enzyme Linked Immuno Sorbent Assay

KP Kanagawa phenomenon

LPS Lipopolysaccharides

PBS Phosphate buffered saline

PCR Polymerase Chain Reaction

PCR- Polymerase chain reaction-restriction fragment length

RFLP polymorphism
PFGE Pulsed fi eld gel electrophoresis
RAPD Random Amplifi ed Polymorphic DNA
Rti-PCR Real-time PCR
TCBS Thiosulfate-Citrate-Bile-Salts Sucrose
U.S.F.D.A. United States Food and Drug Administration

REFERENCES
Abbott, S., Powers, C., Kaysner, C., Takeda, Y., Ishibashi, M., Joseph, S. and Ianda, J. (1989).
Emergence of a restricted bioserovar of Vibrio parahaemolyticus as the predominant cause
of Vibrio -associated gastroenteritis on the West Coast of the United States and Mexico. J.
Clin. Microbiol . 27: 2891-2893.
Abolmaaty, A, Vu, C., Oliver, J. and Levin, R.E. (2000). Development of a new lysis solution
for releasing genomic DNA from bacterial cells for DNA amplifi cation by polymerase
chain reaction. Microbios. 101: 181-189.
Alsallami, A. and Kotlowski, R. (2001). Selection of primers for specifi c detection of
Clostridium botulinum types B and E neurotoxin genes using PCR method. Int. J. Food
Microbiol. 69: 247-253.
Amaro, C. and Biosca, E. (1996). Vibrio vulnifi cus biotype 2 pathogenic for eels, is also an
opportunistic pathogen for humans. Appl. Environ. Microbiol. 62: 1454-1457.
Aono, E., Sugita, H., Kawasaki, J., Sakakibara, H., Takahashi, T., Endo, K. and Deguchi, Y.
(1997). Evaluation of the polymerase chain reaction method for identifi cation of Vibrio
vulnifi cus isolated from marine environments. J. Food Protec . 60: 81-83.
Arias, C., Pujalte, M., Garay, E. and Aznar, R. (1998). Genetic relatedness among environmental,
clinical, and diseased-eel Vibrio vulnifi cus isolates from different geographic regions by
ribotyping and randomly amplifi ed polymorphic DNA PCR. Appl. Environ. Microbiol .
64: 3403-3410.
Badhey, H., Cleri, D., D'Amaato, R., Vernaleo, R., Veinni,V. and Hochstein, L. (1986). Two
fatal cases of type E adult food-borne botulism with early symptoms and terminal
neurological signs. J. Clin. Microbiol. 23: 616-618.