Biology Reference
In-Depth Information
Figure 14.13.
(A) Formation of mixed SAM on Au electrode, (B) immobi-
lization of ferrocene functionalized dendrimers (Fc-D), (C) immobilization
of thiolated capture probe with bifunctional linker, (D) hybridization with
target, (E) hybridization with biotinylated detection probe, (F) association
with avidin-alkaline phosphatase, (G) description of the process of the
electrocatalytic reaction of
p
-aminophenol (
p
-AP) via electronic mediation
of Fc-D.
Incorporation of electroactive ferrocene groups into PAMAM
dendrimers for enhanced electrochemical signal has also been
reported [62]. In this work ferrocene functionalized dendrimers
were immobilized onto a SAM-modified Au electrode, as shown in
Fig. 14.13, and served as an immobilization matrix for the capture
probe DNA, and as an electrocatalyst for
p
-aminophenol oxidation.
p
-aminophenol was produced by alkaline phosphatase labeled
detection probe DNA used in a sandwich-type enzyme-linked DNA
assay. The authors show that use of the ferrocene functionalized
dendrimers lead to a significant enhancement in electrochemical
signalresulting in a 100 pM detection limit.
Gibbs
et al.
[63] reported the formation of dendrimers from
norbornene block copolymers with detection probe DNA and
