Biology Reference
In-Depth Information
withdoxorubicin-modifiedsilvernanoparticles[33].Asdoxorubicin
isawell-knownDNAintercalator,theparticlescouldthenintercalate
into the DNA duplex after hybridization (Fig. 14.5B).
14.2.5.2 Non-stripping-based nanoparticle electrochemical
DNA detection methods
Ruthenium hexamine (RuHex) is a positively charged electroactive
complex that can bind to the anionic phosphate backbone of
DNA strands. Zhang et al. [34] constructed an electrochemical
DNA biosensor by creating a mixed monolayer of DNA probes
onto a gold surface, shown in Fig. 14.6. A sandwich assay was
used to bind DNA-coated gold nanoparticles and bring them in
proximity to the electrode surface. The RuHex marker could then
be bound to DNA strands through electrostatic interactions and
its signal measured as a direct function of DNA hybridization
(Fig. 14.6A). The resulting sensor produced fM detection limits
A: DNA-AuNPs technology
B: Modified bio bar codes technology
C: This method
bridge DNA
Figure 14.6. Schematic diagram for the DNA biosensor fabrication based
on a one-to-one recognition tri-gold nanoparticle DNA probe. And the
comparison of DNA biosensor fabrication based on Au NPs modified with
only one kind of DNA (A: DNA-Au NPs), Au NPs modified with two kinds of
DNA (B: modified bio-bar code technology), and a one-to-one recognition
tri-gold nanoparticle DNA probe technology (C).
 
Search WWH ::




Custom Search