Biology Reference
In-Depth Information
Figure 14.3.
Schematic representation of the immobilization, hybridiza-
tion,and detection of probeDNA.
In another configuration silver nanoparticles were electrode-
posited onto the surface of a previously electro-polymerized
poly(trans-3(-pyridyl) acrylic acid)-multiwalled carbon nanotube,
glassy carbon electrode while DNA hybridization events were mon-
itored by differential pulse voltammetry (DPV) after intercalation
of adriamycin and chemisorption of thiolated single-stranded DNA
onto the silver nanoparticles, as shown in Fig. 14.3 [18]. Multiple
DNAassayswereperformedbyde-hybridizingDNAduplexeswitha
1:1H
2
O:HNO
3
solutionfor15mintoregeneratethesingle-stranded
DNA surface. Both detection schemes showed high sensitivity,
selectivity, and reusability and took advantage of the synergistic
effects of combining carbon nanotubes to increase conductivity
and metal nanoparticles to provide a suitable platform for DNA
immobilization.
14.2.5
Metal Nanoparticle Labels for DNA Hybridization
Detection
14.2.5.1 Direct detection of the nanoparticle label
Costa-Garcia and coworkers [19] first reported on using gold
nanoparticles to electrochemically monitor an a
nity binding
