Biology Reference
In-Depth Information
of user-friendly analysis of interaction of various substances such
as carcinogens, mutagens, or drugs with DNA, according to the
requirements ofpoint-of-care analysis.
The specific determination of interaction between DNA and
related molecules is of impotance in the design of the electrochem-
ical genosensors for application in diagnosis tests and in the design
of new drugs, especially for chemotherapy.
In this chapter, the usage of voltammetric techniques for
compound-DNA interactions were shown with detailed information
which contains some key ways to discover unknown molecule-DNA
interaction mechanisms as electrostatic interactions with the DNA
backbone, covalent or groove binding of the double strand of helix,
and intercalation of aromatic compounds between adjacent base
pairs.
Whencomparedtootheranalysismethodologiessuchassurface
plasmon resonance (SPR), quartz crystal microbalance (QCM)
or impedance (EIS), voltammetry-based sensors provide short
response time, less-expensive analysis about immobilization of
molecules, and in many analyses they allow real-time measure-
ments.
References
1. T. Strachan and A. P. Read,
Human Molecular Genetics
2, 2nd ed., John
Wiley &Sons, BIOS ScientificPublishers Ltd., 1-8 (1999).
2. G. Marrazza, I. Chianella, and M. Mascini, Disposable DNA electrochem-
ical sensor for hybridization detection,
Biosens. Bioelectron
.
14
(1), 43-
51 (1999).
3. E. Palecek,
Topics in Bioelectrochemistry and Bioenergetics
, Vol. 5, John
Wiley,Chichester, 65-155 (1983).
4. E. Palecek, From polarography of DNA to microanalysis with nucleic
acid-modified electrodes,
Electroanalysis
8
(1),7-14 (1996).
5. E. Palecek, Past, present and future of nucleic acids electrochemistry,
Talanta
56
(5),809-819 (2002).
6. E. Palecek, M. Fojta, F. Jelen, and V. Vetterl,
The Encyclopedia of
Electrochemistry, Bioelectrochemistry
, Vol. 9, Wiley-VCH, Weinheim,
365-429 (2002).
