Biology Reference
In-Depth Information
Marrazza
et al.
[71] investigated a electrochemical hybridization
indicator “daunomycin” for detecting Apo E polymorphisms in real
PCR.Wang
et al.
[72]performedthedetectionofinteractionbetween
daunomycin and DNA in the solution phase and at the sensor sur-
face. Erdem and Ozsoz [60] was showed the other electrochemical
redox-active indicator drug “Epirubicin” which was used for the
detection of mismatched sequences. Hashimoto
et al.
[73] obtained
that the anodic signals of daunomycin and doxorubicin shifted to
more positive values after DNA immobilization onto basal plane
pyrolytic graphite transducer.
The changes monitored in the electroactive signals of DNA bases
indicate the behavior of compounds toward DNA [74, 75].
For the investigation of interaction mechanism of some com-
pounds as metal coordination complexes with DNA, Bard
et
al.
[76] reported comprehensive electrochemical studies using
cobalt/ferrum phenanthroline or cobalt/ferrum bipyridine. In that
paper, they evaluated limiting shifts and binding constants of
mediator compounds by cyclic voltammetry in the absence and
presence of DNA in solution phase experiments. As a result of
their report, they found those forms (oxidized or reduced form) of
mediator compounds which bind to the DNA molecule with a high
a
nity.
Carter
et al.
[77] also investigated cobalt phenanthroline and
DNA interactions in their previous paper which contained explana-
tions about the dependence of the redox behavior on the nature of
the ligandscoordinated to the metal center.
Some other examples about drug-DNA interactions have been
seen in the literature. The antibiotic mitomycin C (MC) and its
interactionswithDNAwereinvestgatedbasedonguanineoxidation
signal by Ozkan
et al.
[78]. Meric
et al.
[53] described a biosensor
for the detection of interaction between a compound synthesized
as an alkylating anticancer agent and DNA. Jelen
et al.
[79] found a
redoxactivebis-intercalatoranticancerdrug,Echinomycin,andthey
showeditsinteractionswithDNA.Theintercalator“Adriamycin”and
its
in situ
interaction withDNA was reported by Brett
et al.
[80].
These types of interactions have been reviewed by Palecek and
Fojta [54, 55].
