Biology Reference
In-Depth Information
Physicochemical electrochemical transducers
See
Electrode/working
electrode
Reagent-less detection technique
A procedure that uses no addi-
tional chemical reagents (indicator, redox mediator, enzyme sub-
strate) to generate an analytical signalofthe DNA biosensor.
Redox reaction
A chemical reaction in which the reactants
exchange electrons between each other. As a consequence, the
oxidation states of the elements prior to and following the redox
reaction are altered [66].
Electrode reaction
An interfacial reaction that necessarily involves
a charge transfer step [66] between a chemical reactant (depolar-
izer) and the electrode (an electrochemical reaction). The electrode
reactioninvolvesallprocesses(chemicalreaction,structuralreorga-
nization,adsorption) accompanying the charge transfer step.
Redox mediator
A chemical compound that can shuttle electrons
between two other chemical compounds in solution or between an
electrode and a chemical species in solution [66].
Screen-printed electrode
An electrode prepared by forced screen
printing of a powder-based ink through a screen stencil typically
on a plastic sheet or foil, or ceramic plate, as a single or set of film
electrodes [66].
Selectivity of the DNA-based biosensor
It can be truely considered
as an analytical parameter regarding the analyte detected such as
a specific ssDNA base sequence or protein interacting with nucleic
acid aptamer. Generally, class selectivity to DNA as the recognition
element itself can beconsidered (e.g., at damage to DNA).
Signal-on/signal-off measurement technique
A procedure based
on appearance/diminution of analytical response resulting from
molecular interaction at the biosensor.
Single-/multi-base mismatch
A defect in the double-stranded DNA
structure that distinguishes DNA hybrid containing a mismatched
basepair orpairs from that withfully matched bases.
