Biology Reference
In-Depth Information
scan
Figure 11.7.
LSV and EVLS curves of adenine (Ade), 2-aminopurine (2-
AP), and 2,6-diaminopurine (2,6-DAP) (10
μ
M)with20
μ
M Cu(II) on
PeGE (pencil graphite electrode) in 0.1 M BR buffer, pH 5.1. Reference scan
rate 500 mV/s. Peak Ox
Com
is the anodic signal of Cu (I)-Ade complex,
peak Ox
Ade
is the anodic signal of Ade, accumulation potential
E
a
−
0.15 V,
accumulation time
t
a
120 s. Reproduced with permission from Aladag, N.,
et
al
., Voltammetric study of aminopurines on pencil graphite electrode in the
presence of copper ions,
Electroanalysis
22,
1675 (2010). Copyright Wiley-
VCH Verlag GmbH& Co. KGaA. See also Color Insert.
heights of these peaks. The stability of the accumulated complex
layer was investigated by the AdTS technique. EVLS analysis
using the elimination function E4, eliminating kinetic and charging
currentcomponentsandconservingthediffusecurrentcomponent,
provides the possibility of increasing current sensitivity and of
changing peaks into well-readable peak-counterpeaks (Fig. 11.7).
Fadrna
et al.
[70]hasprovedthatapolishedsilversolidamalgam
electrode, free from liquid mercury, is a suitable substitute for the
HMDE in CSV analyses of purine bases and of acid-treated ODNs.
The analysis was done at nanomol level in alkaline medium in the
presence of Cu(II). Similarly, the application of gold amalgam-alloy
electrode for a sensitive voltammetric detection of ODNs containing
the purine units within the ODN chains in the presence of Cu(II)
