Biology Reference
In-Depth Information
Figure 9.16.
Analytical signals obtained for 50 pM of biotinylated target
with 25% formamide, using a genosensor in which thiolated probes were
immobilizedandstoredat4
◦
Covernight.Reproducedwithpermissionfrom
Wiley InterScience [11].
In order to study the selectivity of the genosensor developed,
hybridization was carried out with 1-, 2-, and 3-base mismatch
complementary strands. When hybridization was performed with-
out applying stringency conditions using a target strand con-
centration of 50 pM, there was no discrimination between the
analytical signals. However, when 25% formamide is added to
a biotinylated target concentration of 50 pM in order to apply
stringency conditions, it is possible to discriminate between the
complementary strand and the 1-, 2-, or 3-base mismatch strands,
as can be seen in Fig. 9.16.
9.4.4
Simultaneous Detection of
Streptococcus
and
Mycoplasma Pneumoniae
Using Gold-Modified
SPCEs
Inthissection,agenosensorforthesimultaneousdetectionoftwoof
the principal causative bacteria of community acquired pneumonia
is developed using a dual screen-printed sensor. The genosensor
design is the same that the used in the previous section (see
Fig. 9.14).
