Biology Reference
In-Depth Information
Figure 9.11.
Ply genosensor response to the complementary target
(ply, white bars), the single-base mismatch target (plymism1, grey bars),
and the three-base mismatch target (plymism3, black bars) for different
concentrations. Data are given as average
3). (a) Enzymatic and
(b) electrocatalytic detection. Reproduced with permission from ACS [29,
54].
±
SD (n
=
base mismatch oligonucleotide sequence is almost the background
signal, indicating that three-base mismatch ply targets can be
perfectly discriminated from the complementary ply target. For the
one-base mismatch oligonucleotide sequence, the analytical signals
obtained only decrease about 30% with respect to those obtained
for the complementary target.
In the optimized experimental conditions the ply genosensor
has been tested for different concentrations of the complementary
oligonucleotide target. In the case of the enzymatic detection, a
linear relationship between peak current and concentrations of
complementary ply target has been obtained between 0.1 and
5pg/
μ
L, with a correlation coe
cient of 0.9993. Thus, these
genosensors can detect 0.1 pg/
μ
L,whichis0.49fmolofplytarget
in 30
μ
L.
