Biology Reference
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Figure 9.10. Schematic representation of the analytical procedure fol-
lowedfortheconstructionofthegenosensorandthedetectionofacomple-
mentary target and a single-base mismatch target. (A) Electrocatalytic and
(B)enzymatic detection. Reproduced with permission from ACS [29, 54].
in very poor manner. Using this method of immobilization of the
oligonucleotide probes, the genosensor devices are stable for a year
iftheyarestoredat4 C.
The ply (pneumolysin sequence) genosensor has been used for
detecting oligonucleotide sequences containing a one- or three-
base mismatch (plymism1 and plymism3, respectively). Three
different concentrations of complementary ply, plymism1, and
plymism3 targets were assayed and three genosensors were used
for each concentration. Figure 9.11 displays the results obtained
with both enzymatic and electrocatalytic detection. For the three
concentrations assayed, the analytical signal obtained for the three-
 
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