Biology Reference
In-Depth Information
Figure 9.9.
Scheme of the DNA self-assembled monolayer formation on
gold electrodes. Reprinted with permission from Elsevier [57].
9.4 Applications
In this section several examples of genosensors based on hybridiza-
tion event, which have been constructed on screen-printed elec-
trodes, will be described. One of them has been designed to detect
a 30-mer SARS (severe acute respiratory syndrome) virus sequence
whilst the others have been designed to identify the nucleic acid
determinants exclusively present on the genome of the pathogen
Streptococcus pneumoniae
.
Although in most of them alkaline phosphatase and 3-indoxyl
phosphate are used as label and enzymatic substrate, respectively,
other label, a platinum (II) complex, will be presented and its
detectiondiscussed.Inallcases,synthetictargetoligonucleotidesas
well as three-base mismatch and one-base mismatch strands of the
pathogen
Streptococcus pneumoniae
or SARS virus are tested using
these genosensor devices. In addition, in the last application of this
section, the versatility of the SPEs design is very useful to carry out
thesimultaneousdeterminationoftwobacteriacausingpneumonia.
