Biology Reference
In-Depth Information
Figure 9.4.
Schematic representation of a impedimetric genosensor
(sandwich hybridization assay). Unmodified PCR products (b) were
captured at the sensor interface (a) via sandwich hybridization with the
surface-tethered probe and a biotinylated signaling probe. The biotinylated
hybrid (c) was then coupled with a streptavidin-alkaline phosphatase
conjugate (d) and finally exposed to the substrate solution (e). The bio-
catalyzed precipitation of an insulating product (f) blocked the electrical
communication between the gold surface and the [Fe(CN)
6
]
3
−
/
4
−
redox
probe (published by Elsevier in Ref. 50).
Figure 9.5.
Scheme of an assay in which enzyme is incorporated through
biotin streptavidin interaction. Reprinted with permission from Elsevier
[49].
