Biology Reference
In-Depth Information
Table 8.1. Metal nanoparticle-based hybridization detection methodsand their limitsof detection
Technique
Detection Method
Target
Detection Limit
Reference
.
ASV detection of Au
III
at SPE
Au-NPlabel on DNA probe
DNA fragment 406 bp
5 pM
[30]
immersedin microwell
0.1
μ
gmL
−
1
(15 nM)
Au-NPlabel on DNA target
Using probe immobilized
DNA fragment 19 bp
[31]
magnetic bead and PSV
detection of Au
III
at SPE
Au-NPlabel on DNA probe
DPVof Au oxidation at graphite
256 bp PCR amplicon
0.78 pM
[32]
pencil electrode
ASV of Ag
I
at carbon fiber electrode
Ag-NPlabel on DNA probe
DNA fragment 32 bp
0.5 pM
[33]
ASV of Cu
2
+
at GCE
Au coated Cu core-shell NPlabelon
Colitoxin
gene 24 bp
5.0 pM
[34]
DNAprobe
Au-NPlabel on DNA target/Agdep.
ASV of Ag
I
at SPE
0.2 ng mL
−
1
(32 pM)
DNA fragment 19 bp
[36]
Au-NPlabel on DNA probe/Ag dep.
DPV of Ag oxidation at GCE
DNA fragment 32 bp
50 pM
[38]
0.2
μ
gmL
−
1
(30 nM)
Au-NPlabel on DNA target/Agdep.
CP detection of Ag at SPE
DNA fragment 19 bp
[37]
LSV of Ag
I
at ITO electrode
Au-NPlabel on DNA probe/Ag dep.
DNA fragment 16 bp
Not given(report greater
[39]
S
=
20 N)
Au-NPlabel on DNA probe/Au dep.
PEG+ NaCl usedin thecatalytic
DNA fragment16 bp
0.6 fM
[40]
process / ASV-CV at SPE
Au-NPlabel on DNA target/Audep.
SWSV at GCE
Primer, wildtypeand mutant
0.35 aM
[41]
DNA of
BRAF
gene 23- 30 bp