Biology Reference
In-Depth Information
Chapter 8
Electrochemical Detection of DNA
Hybridization: Use of Latex to Construct
Metal-Nanoparticle Labels
Mithran Somasundrum
a
and Werasak Surareungchai
b
a
Biochemical Engineering and Pilot Plant Research and Development Unit,
National Center for Genetic Engineering and Biotechnology at
King Mongkut's University of Technology, Thonburi,
Bangkhuntien Campus, Bangkok 10150, Thailand
b
School of Bioresources and Technology, King Mongkut's University of Technology,
Thonburi, Bangkhuntien Campus, Bangkok 10150, Thailand
werasak@pdti.kmutt.ac.th
8.1 Introduction
Of the detection schemes available for DNA biosensors [1], elec-
trochemistry has drawn increasing interest due to enabling high
sensitivities using equipment of relatively low cost. In addition,
electrochemical detection can be coupled readily with available
minaturization technologies [2]. The direct electro-oxidation of
guanine involves high background signals [3], while the use of
enzyme labels may involve deterioration of enzyme activity over
time.Redoxcompoundswhichcanintercalatewiththeprobe-target
