Biology Reference
In-Depth Information
morpholineringsandbondedthroughphosphorodiamidategroups,
resulting in an uncharged nucleic acid. These structures are stable,
highly water soluble, and are cost-effective DNA analogs, which
exhibit improved base stacking compared to PNA analogues. The
utility of LNAs for electrochemical sensing of mismatches remains
tobeexploredbutonecanenvisionthattheresultingduplexshould
exhibit significantly different properties that can be exploited for
sensing.
7.4 Conclusion
Electrochemical detection of DNA mismatches continues to attract
significant attention of the research community. Numerous mis-
match detection schemes have been proposed, some of which even
have led to some limited commercial exploration and start-ups. The
reporteddetectionmethodsvarywidelyfromrelativelysimpleones
that exploit the intrinsic electrochemical properties of DNA and
electric properties of the DNA films, to more complex ones that
employ novel bioconjugates, nanopartiocles and DNA analogues.
This growing interest in electrochemical DNA biosensors is often
driven by the unique advantages offered by the electrochemical
detection methods. Application of electrochemical methods in
a
nity DNA mismatch detection presents likely a promising
alternative for widely used optical methods, potentially allowing
miniaturization with the associated cost reduction, and potential
application in point-of-care assays. Clearly, the future is promising
for electrochemical DNA sensing and much can be expected in the
nextfew years.
References
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2. K.E.Dombrowski,W.Baldwin,andJ.E.Sheats,
J. Organomet. Chem.
281
(1986).
3. A.Okamotoand K.T.I.Saito,
Tetrahedron Lett.
4581
(2002).
