Biology Reference
In-Depth Information
(A)
(B)
(C)
(D)
(E)
Figure 7.12.
Magnetic beads/nanoparticles based protocols for electro-
chemical detection of DNA. These assays involve the introduction of the
probe-coated magnetic beads, addition of the target/hybridization event,
magneticremovalofunwantedmaterials,bindingofthemetalandamplified
electrochemical detection of the dissolved gold (A), silver (B) and cadmium
sulfide (D) nanoparticles. (C) Solid-state stripping and (E) multi-target
detection protocols. Reprinted from
Analytica Chimica Acta
,
500
,J.Wang,
Nanoparticle-based electrochemical DNA detection, 247-257, 2003, with
permission form Elsevier.
compared to complementary target. In addition, this approach does
notdiscriminatebetweendifferenttypes ofmismatches. Wang
et al.
developed a new type of sensor that relies on the mononucleotide
linked nanocrystals, i.e., A-ZnS, C-CdS, G-PbS, and T-CuS that bind
with their complementary nucleotide bases at mismatch sites on
dsDNA modified magnetic bead and thus results in each mutation
withspecific nanocrystal-mononucleotidetags.
