Biology Reference
In-Depth Information
Chapter 5
Electrochemical DNA Biosensors
Marisa Maltez-daCosta,
a
Alfredo de la Escosura-Muniz,
a
and Arben Merko¸i
a,b
a
Nanobioelectronics & Biosensors Group, Institut Catal a de Nanotecnologia,
CIN2 (ICN-CSIC), Esfera Universitat Aut onoma de Barcelona, Bellaterra,
Barcelona, Spain
b
ICREA, Barcelona, Spain
arben.merkoci.icn@uab.es
In this chapter, the use of nanoparticles (NPs) in catalytic
electrochemical analysis of DNA as a new detection strategy
reported in recent years is revised. The topics covered here
include labeling with nanoparticles and their subsequent signal
enhancement employed for DNA hybridization detection. Direct
sensing of nanoparticle labels as well as indirect detection routes
through electrochemical sensing of label-catalyzed reactions have
been reported. Nanofabrication of platforms used for the detection
of DNA through electrochemical signal amplification has also
been revised. Some recent examples of interesting nanoparticle-
induced catalytic methodologies applied for protein detection using
electrochemicalbiosensorsarealsogiven,becauseoftheirpotential
interest in future applications inDNA detection.
