Biology Reference
In-Depth Information
red
Figure 4.7.
Schematic illustration of the amperometric detection of
DNA hybridization based on an enzymatic reaction [39] (reprinted with
permission of the American Chemical Society). See also ColorInsert.
carriedoutonaAu-NP-structuredscreen-printedcarbonelectrode,
and the sequence chosen as target is included in the 29 751-base
genomeoftheSARS(severeacuterespiratorysyndrome)-associated
coronavirus. A linear range was found for the biotinylated target
between 2.5 and 50 pmolL
−
1
with adetection limit of2.5 pmol L
−
1
.
Table 4.2 summarizes works reported between 2007 and 2009
using an enzymatic approach to detect hybridization.
4.3.1.3 Detection based on electrochemical labels
intercalated within dsDNA
DNA sensing platforms constructed with Au-NPs (and other NPs) to
enhancetheDNAimmobilizationandhybridizationperformanceby
increasing the electrode area have also employed for the detection
