Biology Reference
In-Depth Information
Figure 4.2. Dendritic amplification of DNA sensing by oligonucleotide-
functionalized gold nanoparticles.
in nucleic acid tertiary structure [11]. In order to contribute to
a better understanding of the chapter content, we have decided
to separate the covering of immobilization strategies from that of
detection strategies, although, as it will be outlined below, many
immobilization approaches are oriented to achieve amplification of
the resulting analyticalsignals.
4.2 Configurations Used for DNA Immobilization
Asitiswellknown,theimmobilizationofDNAprobesontoelectrode
surfaces is one of the key steps in DNA sensor development. It
has been widely demonstrated that the DNA sensor performance
(e.g., sensitivity, selectivity, and stability) is highly dependent on
the characteristics of DNA probes' immobilization approaches.
One of these relies on the use of nanomaterials such as gold
nanoparticles (Au-NPs), taking advantage of their unique electrical
conductivity, biocompatibility, and ease of self-assembly through
 
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