Biology Reference
In-Depth Information
Figure 3.7. Schematic representation of (A) nanoAu-GEC material show-
ing isolated gold nanoparticles able to produce “bioactive chemisorbing
islands” instead of SAMs on a continuous layer of gold. (B1) Hybridization
assayonthenanoAu-GECelectrode.(B2)Rapidelectrochemicalverification
of thiolated and double-tagged amplicons on the nanoAu-GEC electrode.
Parts C to E are common steps (electrode modification, enzymatic labeling,
andamperometricdetermination)forbothpartsB1andB2(Reprintedwith
permissionfrom Anal.Chem. 2009, 81
,
1332-1339.Copyright2009,Ameri-
can Chemical Society.) See also Color Insert.
0.500:0.500:4 (w/w) for nanoAu(50%)-GEC, and finally, 1:0:4
(w/w) for nanoAu(100%)-EC. The soft paste became rigid after a
curing step of80 Cduring 1 week.
Thereby,thedesignationoftheelectrodesisbasedontheratioof
gold NPstoward graphite particles (the conductive filler).
The location and spatial pattern of the gold NPs on the surface
of the sensor was observed with scanning electron microscopy with
an EDX detector. Figure 3.8, first column, shows, as bright spots,
 
Search WWH ::




Custom Search