Biology Reference
In-Depth Information
When the electrochemical detection is based on enzymatic
activity determination, a capture format was used in which
the immobilization of the biotinylated probe together with the
hybridization was performed in a one-step procedure. The proce-
dureconsistsbrieflyofthefollowingsteps,asschematicallyoutlined
in Fig. 3.5: (i) one-step immobilization/hybridization procedure
Figure 3.5.
Schematic representation of the electrochemical strategy for
the detection of
Salmonella
spp. (A1) One-step procedure based on immo-
bilization of the biotinylated probe onto magnetic beads and hybridization
with the ssDNA target; (A2) rapid verification of PCR amplification based
on the doubly labeled PCR product detection; and (A3) real-time PCR reac-
tor based on PCR amplification with magnetic primers on streptavidin-
modified magnetic beads. Enzymatic labeling (B), magnetic capture of
the modified magnetic beads by the magneto electrode (m-GEC) (C), and
chronoamperometric determination (D) are common steps for all of these
strategies (A-C). (Reprinted with permission from
Biosens. Bioelectron.
22
,
2010-2017 (
2007
).Copyright 2006, Elsevier B.V.). See also Color Insert.
