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the folded nanostructure. Upon addition of equimolar amounts of
the complementary strand (3
) in
an annealing buffer (10 mM Tris•HCl, 0.1 M NaCl, 1 mM EDTA) to
the thermophilic hybrid polymer (
-TAG-GCC-TCA-GTA-GGC-CTA-5
π
5C
), the perylene
-stacking was
0
0
0
1
disrupted, as indicated by the reversal of A
/A
from 0.76 to
0
0
0
1
1.4 (Fig. 5.11). This change of A
/A
ratio reveals complete
π
unfolding of the perylene
-stacks.
1.0
1.0
90 o C
20 o C
unfold
0.5
0.5
0.0
0.0
400
Wavelength (nm)
600
800
400
Wavelength (nm)
600
800
Figure 5.11
Foldamer
binds to complementary DNA and thus unfolds
chromophoric pentamer with the hairpin structures.
5C
The integration of synthetic and natural oligomeric sequences into
a single macromolecule creates folded nanostructures that possess
unusual hyperthermophilic properties. Although temperature is
not efficient at unfolding the thermophilic structure, molecular
recognition can induce unfolding. Such nanoactuation events like
DNA hybridization are useful to construct DNA biosensors based on
color change and such biosensors are not susceptible to temperature
fluctuation.
5.4.2
Hybrid Foldamers as Genes Probes
Detection of DNA has attracted great interest recently, especially in the
area of applying emerging nanotechnology [32
34] to biotechnology
[35
37]. The advantages of integrating nanobiotechnology are that
biological macromolecules such as DNA offer specific interaction
whereas nanotechnologies provide means of amplification of such
molecular recognition events. Typically, a DNA macromolecule binds
to its complementary strand in a subtle different way than that of
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