Biomedical Engineering Reference
In-Depth Information
100
60
20
Pure elastic mass
0
FIGURE 1.38
A
R
representation of the shift values for a series of different biosensors due to ECM remaining on the QCM
surface following complete cell detachment from each of the QCM biosensors. The ECM from ECs are filled cir-
cles; the ECM from MCF-7 cells are unfilled circles. The
f
-
f
-
R
behavior of a pure elastic mass bound at the QCM
surface is indicated by the zero-slope horizontal line. The
R
triangle points determined experimentally for a
series of increasing sucrose concentration solutions is depicted with a best linear fit line. This represents the
behavior of a Newtonian fluid producing a pure (
f
-
R
values.
Reprinted from Marx, K.A., Zhou, T., Montrone, A., McIntosh, D., Braunhut, S.J. (2005). Extracellular Matrix
Studied In Situ With the Quartz Crystal Microbalance: Documenting Cellular Stress During Detachment and the
Elastic Behavior of the Matrix.
Anal. Biochem
. 343:23-34. With permission of Elsevier Publishing.
)
1/2
density-viscosity effect on the QCM
f
-
wound-healing model system. These data are presented in Figure 1.39. Nearly a twofold
acceleration of the wound healing rate was evident by day 9, after only a single adminis-
tration of the solution containing factors released from the ECM (107,108). Since they are
present at a very low concentration, the identity of the active species involved in the accel-
erated wound healing we observed is not currently known. However, experiments are
underway to discover their identity. These experiments involving the intelligent biomate-
rial, ECM, suggest that electrochemical potential stimulation of an ECM-containing
system could be created to accelerate wound healing for use in human injuries.
Experiments are underway to develop this concept, which we term the 'smart bandage',
into a practical device.
1.3
Simulating the Properties of DNA—Potential for Predicting Intelligent
Properties and Evaluating Their Role in Biosensors
1.3.1
Intelligent Properties of DNA
Biological macromolecules, their cellular assemblies, as well as living cells and whole
organisms possess intelligent properties. In this section, we present a discussion focused
on the intelligent properties of the DNA molecule. DNA plays the central role in the
