Biomedical Engineering Reference
In-Depth Information
20
Approaches to Allergy Detection Using Aptasensors
Emile Brys, Sara Tombelli, Maria Minunni, Marco Mascini, and Anthony P. F. Turner
CONTENTS
20.1 Immunoglobulins and Immunoglobulin E ................................................................540
20.1.1 Immunoglobulin E ............................................................................................540
20.1.2 The Hypersensitivity Reaction ........................................................................540
20.1.3 Importance of Immunoglobulin E ..................................................................542
20.1.4 Current Diagnosis Methods ............................................................................543
20.1.5 Affinity Biosensors ............................................................................................544
20.1.5.1 Types of Affinity Biosensor ..............................................................544
20.1.5.1.1 Immunosensors ..............................................................544
20.1.5.1.2 Other Types of Affinity Biosensors ..............................546
20.1.6 Recent Work on Immunoglobulin E Biosensors ..........................................546
20.2 Surface Plasmon Resonance ........................................................................................547
20.2.1 Theory ................................................................................................................547
20.2.2 Concept ..............................................................................................................549
20.2.3 Applications ......................................................................................................549
20.2.4 Future Developments ......................................................................................551
20.3 Aptamers ........................................................................................................................552
20.3.1 Aptamer Libraries ............................................................................................552
20.3.2 SELEX ..................................................................................................................553
20.3.3 Aptamers and Antibodies ................................................................................555
20.3.4 Applications ......................................................................................................557
20.3.4.1 Two Site Binding Assays ..................................................................557
20.3.4.2 Flow Cytometry ................................................................................557
20.3.4.3 Biosensors ..........................................................................................557
20.3.4.4 Molecular Beacons ............................................................................558
20.3.4.5 Capillary Electrophoresis ................................................................559
20.3.4.6 Molecular Switches ..........................................................................559
20.3.4.7 Aptamer Arrays ................................................................................560
20.3.4.8 Aptazymes ..........................................................................................560
20.4 Implementation and Illustration of Instrumentation ..............................................561
20.4.1 Immobilization ..................................................................................................561
20.4.2 Calibration ..........................................................................................................561
20.5 Future Prospects ............................................................................................................562
References ....................................................................................................................................562
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