Biomedical Engineering Reference
In-Depth Information
(A)
(B)
(C)
FIGURE 1.25
(See color insert following page 330)
Light micrographs of Coomassie blue-stained ECs attached to the
surface of three different electropolymerized films: (A) poly-tyrosineamide film, (B) poly-RGDY:tyrosineamide
film (1:20 molar ratio), (C) poly-RGDY:tyrosineamide film (1:3 molar ratio). Reprinted from Marx, K.A., Zhou,
T., McIntosh, D., Braunhut. S.J. (2004). Electropolymerization of Biomimetic Peptide-Tyrosineamide Polymer
Films for Specific Cell Attachment.
Arch. Appl. Biomater. Biomol. Mater., Mater. Res. Soc.
EXS-1:169-171. With
permission of the Materials Research Society.
RE (Ag/AgCl)
CE (Pt)
E
I
F
EG&G 273
potentiostat
Computer
WE
QCA 917
main unit
Oscillator
O-ring
WE
QCM crystal
F
A
Computer
H
2
O
FIGURE 1.26
Electrochemical quartz crystal microbalance device schematic is presented. This electrochemical set-up comprised
the three-electrode configuration and associated potentiostat for computer-controlled electrosynthesis used to
electropolymerize a number of films. The oscillator was used for measuring the
R
of the oscillating quartz
crystal during and following film synthesis and in studies of biosensor applications, including growing cells upon
films following their synthesis as in Figures 1.24 and 1.25. For the cell growth application, we have shown the EQCM
device placed inside a cell culture incubator. Reprinted from Marx, K.A., Zhou, T., McIntosh, D., Braunhut. S.J. (2004).
Electropolymerization of Biomimetic Peptide-Tyrosineamide Polymer Films for Specific Cell Attachment.
Arch. Appl.
Biomater. Biomol. Mater., Mater. Res. Soc.
EXS-1:169-171. With permission of the Materials Research Society.
f
and
1.2.3.1 Quartz Crystal Microbalance Biosensor Detects Enzymatic Polymerization
We have studied both electrochemical and enzymatic polymerization and the thin-film
properties of a number of biopolymer systems. Some examples of these we discussed in the
prior section on electrochemical signal transduction methods. For one of these systems, the
decyl ester derivatives of
D
-tyrosine (DEDT) shown in Figure 1.22 (85), we have also shown
that the QCM can act in a novel way as a sensitive biosensor of enzymatic polymerization
taking place in solution and on the gold electrode surface above the oscillating quartz crys-
tal. We had previously demonstrated that these amphiphilic monomers self-assemble into
