Biomedical Engineering Reference
In-Depth Information
50
50
1:3 RGDY:tyr-am
40
0
30
50
20
100
10
R
150
0
10
200
20
250
f
30
300
40
50
350
0
500
1000
1500
2000
2500
3000
3500
t (s)
300
450
1:3 RGDY:tyr-am
Cells added
400
100
350
R
f
100
300
250
300
200
500
150
100
700
50
f
900
0
R
50
1100
0
5
10
15
20
t (h)
FIGURE 1.24
Time course of film formation is presented in the upper panel measured via the
R shifts of an electro-
chemical quartz crystal microbalance (EQCM) device during electropolymerization by a series of cyclic voltam-
metry (0-1.0 V) sweeps of the comonomer mixture, tetrapeptide Arg-Gly-Asp-Tyr (RGDY) and tyrosineamide in
a 1:3 ratio. In the lower panel, the time course of endothelial cell (EC) binding to the electropolymerized film is
presented for 20,000 cells added at the time indicated by arrowhead following a 2 h baseline signal with only
media and serum.
f and
in a three-electrode electrochemical cell. This set-up can sensitively detect changes in mass
deposition down into the nanogram range via changes in the resonant frequency, f , and
motional resistance, R , of the oscillating quartz substrate. Originally developed for gas-
phase use, in the early 1980s solution-phase QCM was developed and has been applied to
a number of fundamental studies of biomolecular systems, polymeric systems and whole
cells. For a detailed discussion, we refer the reader to an extensive recent review from the
Center for Intelligent Biomaterials on the use of the QCM and EQCM in solution-based
biosensor and biomaterials characterization applications (65).
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