Biomedical Engineering Reference
In-Depth Information
1.8
1.6
1.4
1.2
1.0
0.8
0.6
0.4
0.2
0.0
T205A
E204Q
T205A,N202S
124 0 5 060
200 750 1K 2K 4K 10K 15K 25K 40K
Concentration of 1,2 diaminopropane (ppm)
100
400
(a)
2.4
2.2
2.0
1.8
1.6
1.4
1.2
E204Q (TEA)
E204Q (12DAP)
1.0
0.8
0.6
0.4
0.2
0.0
WT (TEA)
WT (12DAP)
1
2
4
10
15
30
60
100
200
400
750 1K
2K
4K 10K 15K 25K 40K
(b)
Concentration (ppm)
FIGURE 14.10
(See color insert)
The sensitivity of wild-type BR and mutants in the presence of several chemical antigens is illus-
trated. Sensitivity is defined as the ratio of the change in the M/O decay time constant to the change in micromolar
concentration of the added chemical. (a) Sensitivity to 1,2-diaminopropane (1,2-DAP) varies between the wild-type
protein and BR mutants. All of the mutants demonstrated better sensitivity than wild-type BR. (b) Not only sensi-
tivity to one chemical varies between proteins as in (a), but the same protein responds differently from one chemical
to another. This is illustrated for the wild-type protein and the mutant E204Q, for 1,2-DAP and triethylamine.
