Biomedical Engineering Reference
In-Depth Information
(a)
(b)
(c)
(d)
(e)
FIGURE 6.4
Dual-array MEAs compatible with the regular amplifier contact geometry. (a) 5
5 cm plate showing recording
site, contact circle of 'O'-rings (dashed lines), and gasket holding medium during network development. The
inner circles represent the cell adhesion areas. (b) Electrode configuration of one recording site showing 32 cru-
ciform electrodes separated by 200 µm laterally and between rows. (c) Top and bottom views of recording cham-
bers. (d) Assembled chamber and base plate with heating power resistors. (e) Multipolar neuron near cruciform
electrode terminals.
(c)
(a)
(b)
FIGURE 6.5
Plexon multichannel data analysis display. (a) A selected channel shows action potential waveshapes on
that channel and allows setting of templates for real time discrimination (b) Separation into logical time-
stamp channels. (c) All discriminated units, selected by template matching algorithms, are shown in the right
panel.
confirmed by a second, higher threshold (T2). In this manner small bursts of 3-4 spikes can
be eliminated if emphasis is to be placed on larger bursts. Burst identification is very much
an operational process and depends on the type of activity pattern generated by the
cultures.
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