Lentiviral Vectors for Gene Delivery to the Nervous System - Gene Delivery and Therapy for Neurological Disorders - page 39

Table 4
(continued)

Reagents and consumables Sources and catalog numbers

Opti-MEM ® I reduced serum medium,
no phenol red

Gibco, Life Sciences, # 11058021

Phosphate buffer saline (10×) SIGMA, #D8537

ProLong gold antifade reagent Invitrogen, P36930

Poly- L -lysine coated slides VWR, 631-0107

Polypropylene ultracentrifugation tube Beckman, 337986

Retro-Tek HIV-1 p24 antigen ELISA kit Zeptometrix, 0801200

Screw top centrifuge tubes Nalgene

Triton X-100 SIGMA, X100

Trypsin-EDTA SIGMA, #T4174

0.20 ʼ m Filter (500 ml capacity) Thermo Scientifi c, 162-0020

0.45 ʼ m Filter (500 ml capacity) Thermo Scientifi c, 162-0045

0.20 ʼ m Syringe fi lter Nalgene, 190-2520

0.45 ʼ m Syringe fi lter Nalgene, 190-2545

T175 cm 2 fl asks Nunc, 159910

T75 cm 2 fl asks Nunc, 156499

12-Well plate Nunc, 150628

24-Well plate Nunc, 142475

150 × 25 mm Tissue culture dish BD Falcon, 353025

UV half area plate, 96 well Corning, 3679

Vybrant multicolor cell-labeling kit (DiO,
Dil, DID solutions)

Life Sciences, #V22889

pRRLsin - cPPT - CMV-eGFP - WPRE Transgene cassette contains a self-inactivating

3

LTR. eGFP
Reporter gene driven by CMV promoter, also codes
for ampicillin resistance

′

pMD2 - LgpRRE Structural protein is within a pMD2 plasmid backbone

carrying an ampicillin resistance gene and driven by a
Cytomegalovirus (CMV) promoter

pRSV - Rev Accessory protein situated within plasmid carrying

ampicillin resistance gene and driven by the RSV U3
promoter

pMD2 - VSVg VSV envelope glycoprotein driven by a CMV promoter

and encodes for ampicillin resistance gene.