Biomedical Engineering Reference
In-Depth Information
3
Infusion of AAV Vector into the Brain of Large Animal Models of LSDs
3.1 Materials
and Reagents
Drugs
Atipamezole (Antisedan, Zoetis)
Buprenorphine (Buprenex, Reckitt Benckiser)
Dexmedetomidine (Dexdomitor, Zoetis)
Florfenicol (Nufl or, Schering-Plough)
Flunixin meglumine (Banamine, Schering-Plough)
Isofl urane (Isothesia, Butler Animal Health Supply)
Ketamine (Ketaset, Fort Dodge)
Lactated Ringer's Solution
Midazolam (Hospira Inc.)
Morphine (West-Ward)
Injection contrast reagent
Gadoteridol (Prohance, Bracco Diagnostics, 279.3 mg/mL)
Equipment
Intravenous catheter (Surfl ash, Terumo)
Rectal temperature probe (ReliOn)
Electrocardiogram (Cardiocap II, Datex-Ohmeda)
Capnograph (Capnomac Ultima, Datex-Ohmeda)
Pulse oximeter (Cardiocap II, Datex-Ohmeda)
Doppler peripheral pulse monitor (Parks Medical)
Sphygmomanometer (Parks Medical)
Veterinary patient warming system (Hot Dog, Augustine
Biomedical Design)
Horsley-Clarke stereotaxic apparatus (David Kopf Instruments)
Hamilton syringe (Harvard Apparatus)
Non-coring needle (22-25 G) (Harvard Apparatus)
8-5 MHz ultrasound probe (Philips HDI 5000)
4-0 monofi lament suture (Monocryl, Ethicon)
Oscillometric
blood
pressure
monitor
(Cardiocap
II,
Datex-Ohmeda)
0 monofi lament suture (PDS II, Ethicon)
3.2 Stereotaxic
Injection of AAV
Vectors into the Brain
of Feline Models
of Gangliosidoses
Feline models of GM1 and GM2 gangliosidosis were fi rst described
in the 1970s [
92
,
93
] and have been extremely well characterized
in the intervening years [
94
,
95
], presenting an unparalleled
opportunity to test therapeutic options for translation to human
clinical trials. The cat brain is only ~16 times smaller than the infant
human brain, which it closely resembles in terms of complexity and
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