Biomedical Engineering Reference
In-Depth Information
been demonstrated by both improved survival and behavior in a
multitude of mouse models for LSDs with nervous system involve-
ment [
45
,
47
,
74
,
83
]. Gene transfer to the CNS in mice and
humans was fi rst achieved using rAAV2 serotype [
84
]. Since then,
other more effi cient serotypes for CNS gene transfer have been uti-
lized such as rAAV1, rAAV5, rAAV8, rAAV9, rAAVrh8, and
rAAVrh10 [
41
,
65
,
66
,
69
,
71
]. Most recently, rAAV9 and other
capsids have been shown to effi ciently transduce the CNS through
systemic delivery via intravenous injection in adult mice and provide
another potential approach for gene therapy for lysosomal storage
disorders [
67
,
72
,
73
].
Stereotaxic instrument; Stoelting 51730D
Injector; World Precision Instruments; UltraMicroPump III
Syringe pump controller; World Precision Instruments #SYS-MICRO4
Gastight syringe, 10
2.1.1 Materials
and Reagents
L*; Hamilton #7653-01
33 gauge steel needles*; Hamilton #7762-06
Sterile 0.5 M sodium hydroxide
Sterile water
1 mL syringes with permanent needle; Kendall Monoject
#1188128012
Ketamine (100 mg/mL)
Xylazine (20 mg/mL)
0.9 % sodium chloride; Hospira #0409-4888-10
Puralube
®
ophthalmic ointment; Webster #07-888-2572
Small electric shaver
70 % isopropyl alcohol prep pads
Povidone-iodine prep pads
Sterile disposable scalpels; Dynarex #4111
Sterile cotton swabs; Puritan #25-806 1WC
Small drill bits*
Handheld drill
Stereotaxic atlas
Sterile AAV vector to be injected
Refl ex™ clip applier*
Refl ex™ clips, 9 mm*
Refl ex™ clip removing forceps*
Warm water recirculation pump
Warm water circulation pad
Ketoprofen (100 mg/mL)
*Autoclave before use
ʼ
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