Gene Therapy for Epilepsies - Gene Delivery and Therapy for Neurological Disorders - page 246

DNAse I
Tissue culture grade distilled water
Proteinase K
TE buffer, pH 8.0
2× SYBR Green mix
Forward primer
Reverse primer
Thermal cycler (PCR machine) 96-well with hot top
2-ml microfuge tubes
96-well optical reaction plate
Optical adhesive cover
Plate sealers
Centrifuge with 96-well plate rotor
Roche LightCycler ® 480 system or an equivalent real-time PCR
system

2.3 Rodent
Stereotaxic
Neurosurgery

Rodent brain atlas
Stainless surgical instruments (scalpel, forceps, locked forceps)
Disposable surgical blade
Stereotaxic frame (e.g., Model 900 Small Animal Stereotaxic,
David Kopf Instruments, Tujunga, CA, USA)

Electric dental drill
Microinfusion pump (e.g., Micro4 microsyringe pump controller,
World Precision Instruments Inc., Sarasota, USA)
Light source (e.g., Fiber-Lite high-intensity illuminator, Dolan-
Jenner Industries)
Hamilton syringe (Model 1701 N SYR, 26 s G)
Electric hair shaver
70 % ethanol
Distilled water
Betadine
Ketamine hydrochloride
Xylazine
Eye ointment
Permanent fine-tip marker
Disposable 26-G needle
0.5-cc insulin syringe, with 28-G needle
Cotton-tipped applicators
4-0 polydioxanone suture
Clean cage
Paper towel
Heating pad

In addition to the materials required for neurosurgery listed above
(except for the micropump system and suture), the following
materials are needed for KA seizure models:
Insulated nichrome wire (e.g., formvar-insulated nichrome
wire, A-M Systems, Sequim, WA, USA)
Blade (for removing insulation)

2.4 Seizure Models