Biomedical Engineering Reference
In-Depth Information
Fig. 2
Location and exposure of the L4/L5 dorsal root ganglia. (
a
) Location of the
L4 and L5 DRG with respect to the level of the iliac crest (
dotted line
). The overly-
ing vertebrae (L4-L6) are shown as semi-transparent. The
boxed area
(L5 verte-
bra and DRG) is shown in panels (
b
-
e
). (
b
,
c
) Exposure begins by removing the
L5 caudal articular process (1) and accessory process (2). (
d
) The L6 cranial
articular process (3) is exposed and laminectomy of the L5 vertebra continues
rostrally. Connective tissue (
asterisk
) may cover the DRG at this point. (
e
) The
connective tissue is removed and the L6 cranial articular process is partially
removed to complete the exposure of the L5 DRG. The L4 DRG should then be
exposed in the same way before injecting both ganglia with viral vector
The epineurium can be opened by picking it with a 30 G
needle (mounted on a 1 ml syringe to hold). An opening should be
made where the needle will be inserted and needs to be only large
enough for this.
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