Biology Reference
In-Depth Information
A
B
C
120
100
Group I
Group II
Normal
Blind
Group I
Group II
Normal
Blind
***
100
80
80
60
60
40
40
20
20
0
0
Latency
Time on
shallow end
0-50
50-80
>80
Time on shallow end (s)
D
E
F
100
0.42
90
Group I
Group II
Normal
Blind
0.40
80
Group I
Group II
Normal
Blind
*
0.06
70
0.04
20
0.02
10
0.00
0
3
6
8
10-12
£
0.08
0.08-0.16
³
0.16
Time (weeks)
OMR, cycles/degree
Individual mice
Group averages
G
7 PM
0.03
0.02
0.01
0.00
0.03
0.02
0.01
0.00
0.03
0.02
0.01
0.00
7 AM
7 PM
7 AM
7 PM
7 AM
7 PM
7 AM
7 PM
7 AM
7 PM
7 AM
7 PM
7 AM
7 PM
7 AM
7 PM
7 AM
7 PM
7 AM
0.03
0.02
0.01
0.00
0.03
0.02
0.01
0.00
0.03
0.02
0.01
0.00
Normal
Group I
Group II
0.03
0.03
0.02
0.01
0.00
0.02
0.01
0.00
Blind
Figure 6.5 Partial recovery of function. Tests for three visual responses in normal mice
(black bars) and in mice with optic nerve injury and the pten gene deleted (Group I, blue
bars) or present (Group II, red bars). Mice in Groups I and II both received intraocular
inflammation combined with CPT-cAMP. (A) Visual cliff apparatus used to evaluate
depth perception. (B) Left, mice with optic nerve damage show a tendency to step
off the shallow end faster than normal animals. Right, total time spent on shallow
end. Group I mice, like normal controls, show a preference to return to the shallow
end. ***P
<
0.01. (C) Histogram showing distribution of population from each group
and the time spent on shallow end. (D) Apparatus used to evaluate optomotor response
(OMR). (E) Average OMR (response threshold, cycles/degree) as a function of time.
Note improvements in Group I. (F) Frequency distribution of the OMR. The y-axis in
F is discontinuous. (G) Circadian photoentrainment: left, percent of overall activity in
1-h bins for individual mice; right, group averages. Mice were maintained on a contin-
uous cycle of lights on at 7 AM and off at 7 PM prior to testing and for the first 2½ days in
the activity monitor. The light cycle was set back 6 h on day 3. Error bars represent SEM.
