BIOAUGMENTATION FOR THE IN SITU AEROBIC COMETABOLISM OF CHLORINATED SOLVENTS - Bioaugmentation for Groundwater Remediation - page 225

Table 8.3. Microcosm and Column Studies of the Bioaugmentation Process

Bioaugmentation
Process

Substrate or Cells
Added

Study Type of Study Culture Added Contaminant Added

Hanna and Taylor
( 1996 )

Approach I
Resting cell
biocatalysis activity

Continuous flow
column

Methylosinus
trichosporium OB3b

TCE Resting cells

10 9
cell/g dry sand)

10 8 -1.5 (7

Tompson et al. ( 1994 ) Approach I

Resting cell
biocatalysis activity

Continuous flow
column

Methylosinus
trichosporium OB3b

TCE (4 mg/L) Resting cells

(2 10 8 cell/g dry sand)

Munakata-Marr et al.
( 1996 , 1997 )

Approaches I and II
Biocatalysis activity
Strain with
constitutively
expressed oxygenase

Batch exchanged
column
Aquifer/groundwater

Burkholderia cepacia
G4
B. cepacia PR1 23
and PR1 301

TCE (250 m g/L) Lactate (15 mg/L)

Phenol (6.5 mg/L)
70 m g of cells

10 7 cells/mL
Phthalate (4 mM)

Synder et al. ( 2000 ) Approach I and II

Resting cell
and substrate

Continuous flow
column

Burkholderia cepacia
G4

TCE (66-100 g/L) 6.3-6.6

m

B. cepacia 5.7 10 10
CFU
K. oxytoca 1.6 10 11
CFU
30 to 70 mg/L DOC

Komlos et al. ( 2004 ) Approach II

Biofilm cometabolism

Continuous flow
column

Burkholderia cepacia
PR1 and Klebsiella
oxytoca

TCE (1.1-1.2 mg/L)

Jitnuyanont et al.
( 2001 )

Approach III
Strain with enhanced
transformation abilities

Batch slurry
Aquifer/groundwater

Mixed culture 1,1,1-TCA (50-250 m g) Butane (5 mg)

(continued)