Biomedical Engineering Reference
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Figure 6.6. (a) Increase in fluorescence intensity ( D Rn) at each cycle number. The cycle threshold is
indicated by the line at 0.2. (b) The cycle at which the fluorescence intensity crosses the cycle threshold
is plotted against the gene copies for each standard, to generate a standard curve for use in quantifi-
cation of a gene target. Courtesy of Kirsti Ritalahti and Frank L¨ ffler, University of Tennessee.
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