Biomedical Engineering Reference
In-Depth Information
7.8
7.6
7.4
Nitrogen sparging
7.2
7
6.8
6.6
6.4
0
3
5
8
10
13
16
Time (d)
Figure 3.7. Effect of nitrogen sparging on pH during growth of the PJKS
TM
culture. YE (0.1%) was
added at the beginning of the fermentation, and again on day 16 (0.05%). Sodium lactate (60%
solution) feeding began on day 2 at a rate of 21.6
m
L/h
3
L, and it was increased to 24
m
L/h
3
Lon
day 3 and to 28.8 mL/h 3 L on day 11.
7.1
7
6.9
6.8
6.7
6.6
6.5
6.4
6.3
0
5
10
15
20
25
Time (min)
Figure 3.8. Effect of nitrogen sparging on the pH of an SDC-9
TM
culture in a 750-L reactor at 28
o
C.
These tests were performed to evaluate the duration of nitrogen sparging required to maintain a
desirable pH for Dhc growth. The OD
550
of the culture at the time of sparging was 1.1, and nitrogen
was added at the rate of 12-14 L/min or 4.4-4.8 mL/min 3 L medium.
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