DEHALOCOCCOIDES AND REDUCTIVE DECHLORINATION OF CHLORINATED SOLVENTS - Bioaugmentation for Groundwater Remediation - page 68

Table 2.7. (continued)

Dehalococcoides
16S rRNA Gene -
Targeted

Probe/
Primer

Nucleotide
Position Sequence (5 0 -3 0 )

Chemistry Reference

All known
dehalogenating
chloroflexi

Chl348F SYBR green GAGGCAGCAGCAAGGAA 348-364 (Fagervold et al., 2005 )-Chloroflexi

specific

Dehal884R SYBR green GGCGGGACACTTAAAGCG 867-884 Fagervold et al., 2005 : Dehalogenating

Chloroflexi including Dehalococcoides
and DF-1

bvcA gene bvcAF TaqMan AAAAGCACTTGGCTATCAAGGAC 1,011-1,033 Krajmalnik-Brown et al., 2004 ; Ritalahti

et al., 2006

bvcAR TaqMan CCAAAAGCACCACCAGGTC 1,076-1,058 Krajmalnik-Brown et al., 2004 ; Ritalahti

et al., 2006

bvcAP TaqMan TGGTGGCGACGTGGCTATGTGG 1,035-1,056 Krajmalnik-Brown et al., 2004 ; Ritalahti

et al., 2006

bvcA gene Forward TaqMan GGTGCCGCGACTTCAGTT 73-90 Holmes et al., 2006 ; Johnson et al., 2005

Reverse TaqMan TCGGCACTAGCAGCAGAAATT 140-120 Holmes et al., 2006 ; Johnson et al., 2005

Probe TaqMan TGCCGAATTTTCACGACTTGGAT

GAAG

92-118 Holmes et al., 2006 ; Johnson et al., 2005

vcrA gene vcrAF TaqMan CGGGCGGATGCACTATTTT 400-418 Ritalahti et al., 2006

vcrAR TaqMan GAATAGTCCGTGCCCTTCCTC 471-451 Ritalahti et al., 2006

vcrAP TaqMan CGCAGTAACTCAACCATTTCCTGG

TAGTGG

420-449 Ritalahti et al., 2006

vcrA gene Forward TaqMan CTCGGCTACCGAACGGATT 1,303-1,321 Holmes et al., 2006

Reverse TaqMan GGGCAGGAGGATTGACACAT 1,367-1,348 Holmes et al., 2006 ; Johnson et al., 2005

Probe TaqMan CGCACTGGTTATGGCAACCACTC 1,345-1,323 Holmes et al., 2006

vcrA gene Forward SYBR green TGCTGGTGGCGTTGGTGCTCT 627-647 M¨ ller et al., 2004

Reverse SYBR green TGCCCGTCAAAAGTGGTAAAG 1,067-1,047 M ¨ ller et al., 2004

(continued)