DEHALOCOCCOIDES AND REDUCTIVE DECHLORINATION OF CHLORINATED SOLVENTS - Bioaugmentation for Groundwater Remediation - page 67

Table 2.7. Primers and Probes Used for the Specific Detection/Quantification of Dhc and Dechlorinating Dhc Relatives
Dehalococcoides
16S rRNA Gene -
Targeted

Probe/
Primer

Nucleotide
Position Sequence (5 0 -3 0 ) Chemistry Reference

Dehalococcoides
strains

Dhc1200F TaqMan CTGGAGCTAATCCCCAAAGCT 1,200-1,220 He et al., 2003a , b

Dhc1271R TaqMan CAACTTCATGCAGGCGGG 1,265-1,248 He et al., 2003a , b

Dhc1235P TaqMan TCCTCAGTTCGGATTGCAGGCTGAA 1,222-1,246 He et al., 2003a , b

Dehalococcoides
strains

Forward TaqMan GGTAATACGTAGGAAGCAAGCG 467-488 Holmes et al., 2006 ; Rahm, 2006

Reverse TaqMan CCGGTTAAGCCGGGAAATT 564-546 Holmes et al., 2006 ; Rahm, 2006

Probe TaqMan ACATCCAACTTGAAAGACCACCTAC

GCTCACT

543-512 Holmes et al., 2006 ; Rahm, 2006

Dehalococcoides
strains

Dhc1f SYBR green GATGAACGCTAGCGGCG 1-17 Duhamel et al., 2004 ; Hendrickson et al.,

2002 ; Duhamel and Edwards, 2006 ;
Duhamel and Edwards, 2007 ; United
States Patent US6894156B2
(Hendrickson and Ebersole)

(Dupont primer set 1)

Dhc259r SYBR green CAGACCAGCTACCGATCGAA 259-240 Duhamel et al., 2004 ; Duhamel and

Edwards, 2006 ; Duhamel and Edwards,
2007; Hendrickson et al., 2002 ;
United States Patent US6894156B2
(Hendrickson and Ebersole)

Dehalococcoides
strains

Forward SYBR green GAAGTAGTGAACCGAAAGG 240-259 Schaefer et al., 2009

Reverse SYBR green TCTGTCCATTGTAGCGTG 1,176-1,159 Schaefer et al., 2009

Dehalogenimonas
strains

BL-DC-631f SYBR Green GGTCATCTGATACTGTTGGACTTGA
GTATG

634-663 Yan et al., 2008

BL-DC-8 and BL-DC-9 BL-DC-796r SYBR green ACCCAGTGTTTAGGGCGTGGACTA

CCAGG

799-827 Yan et al., 2008

(continued)