Biology Reference
In-Depth Information
Growth factor(s)/
neuroprotective
molecules
Gap
size
(mm) Methods
Follow-
up
Authors
and year
Nerve tube
Internal filler
Animal
Injury
Controls
Outcome
Laminin-
coated
chitosan
tube
Nogo-66
receptor protein,
basic fibroblast
growth factor
(bFGF),
epidermal
growth factor
(EGF), and
platelet-derived
growth fator
(PDGF)
NSPCs preseeded
on laminin-
coated chitosan
tube in
combination
with nogo-66
receptor protein,
basic fibroblast
growth factor
(bFGF),
epidermal growth
factor (EGF), and
platelet-derived
growth fator
(PDGF)
Female
Sprague-
Dawley
rats
Complete spinal
cord transection
T7-T9 laminae
were removed,
the facets at the
same levels were
removed and the
dura mater was
longitudinally
incised in the
midline and then
excised at T8
-
Basso-Beattie-
Bresnahan (BBB)
test and
histological
evaluation
Chitosan
tube without
growth
factors or
nogo-66
receptor
protein
12 weeks The combination
of nogo-66
receptor protein,
growth factors,
and NSPCs
increases survival
of transplanted
NSPCs and
enhances axonal
regeneration
Guo
et al.
(2012)
Chitosan
tube
Dibutyryl cyclic-
AMP
Poly-lactic-
co
-
glycolic acid
(PLGA)
microspheres
containing
dibutyryl cyclic-
AMP (dbcAMP)
in combination
with NSPCs
Female
Sprague-
Dawley
rats
Complete spinal
cord transection
laminectomy was
performed on
T7-T9 exposing
the spinal cord.
The facets of the
vertebrae at T7-
T9 were also
removed
-
Functional and
histological
evaluation
Chitosan
tube
containing
untreated
NSPCs
6 weeks
dcb-AMP
treatment results
in greatest
number of
NSPCs
differentiated
into neurons.
Combination of
NSPCs with
chitosan tube
results in
extensive axonal
regeneration into
the injury site and
improvement of
functional
recovery
Kim
et al.
(2011)
