Biology Reference
In-Depth Information
Table 1.3 Types of supportive cells used to enhance chitosan tubes in spinal cord repair
Combination with cells
Gap
size
(mm) Methods
Nerve
tube
Follow-
up
Authors
and year
Cell type
Internal filler
Animal
Injury
Controls
Outcome
Laminin-
coated
chitosan
tube
Neural
stem/
progenitor
cells
(NSPCs)
NSPCs preseeded
on laminin-coated
chitosan tube in
combination with
nogo-66 receptor
protein, basic
fibroblast growth
factor (bFGF),
epidermal growth
factor (EGF), and
platelet-derived
growth fator
(PDGF)
Female
Sprague-
Dawley
rats
Complete spinal
cord transection
T7-T9 laminae
were removed, the
facets at the same
levels were
removed, and the
dura mater was
longitudinally
incised in the
midline and then
excised at T8
-
Basso-Beattie-
Bresnahan (BBB)
test and
histological
evaluation
Chitosan
tube
without
growth
factors or
nogo-66
receptor
protein
12 weeks The combination
of nogo-66
receptor protein,
growth factors, and
NSPCs increases
the survival of
transplanted
NSPCs and
enhances axonal
regeneration
Guo et al.
(2012)
Chitosan
tube
Bone
marrow
stromal
cells
(BMSCs)
-
Rats
Complete spinal
cord transection
-
Basso-Beattie-
Bresnahan (BBB)
test, retrograde
tracing, and
histological
evaluation
Chitosan
tube alone
12 weeks Enhanced axonal
regrowth,
remyelination, and
functional recovery
Chen et al.
(2011)
Poly-lactic-
co
-
glycolic acid
(PLGA)
microspheres
containing
dibutyryl cyclic-
Chitosan
tube
Neural
stem/
progenitor
cells
(NSPCs)
Female
Sprague-
Dawley
rats
Complete spinal
cord transection
laminectomy was
performed on T7-
T9 exposing the
spinal cord. The
-
Functional and
histological
evaluation
Chitosan
tube
containing
untreated
NSPCs
6 weeks
dcb-AMP
treatment results in
greatest number of
NSPCs
differentiated into
neurons.
Kim et al.
(2011)
Continued
