Biology Reference
In-Depth Information
Table 1.2 Modifications to chitosan conduits surface used in central nervous system repair
Surface modification
Gap
size
(mm)
Authors
and year
Surface
modification
Internal
filler
Nerve tube
Animal
Injury
Methods
Controls
Follow-up Outcome
Chitosan
tube
Collagen
type-I
Semifluid
collagen
type-I
Wistar
rats
Laminectomy from
T7 to T10
vertebrae
รพ
lateral
incision at T9 to
excise a segment of
spinal cord of 4 mm
length and 2 mm
width
About
two-
third
spinal
cord
Basso-Beattie-
Bresnahan (BBB)
test, anterograde
axonal tracing with
biotin dextran
amine (BDA),
retrograde axonal
tracing with
FluoroGold (FG),
and histological
evaluation
Empty
chitosan
tube and
lesion
without tube
implantation
12 months Axons from the
proximal spinal cord
regenerate, cross the
lesion area inside the
tube, and lead to
functional
restoration of
paralyzed hind limbs
Li,
Yang,
Zhang,
Wang,
and
Chen
(2009)
Porous
chitosan
tube
Laminin
coating of the
inner surface
by oxygen
plasma
treatment
-
Female
Sprague-
Dawley
rats
Complete
transection at T8
and removal of
5 mm piece
of spinal
cord tissue
5
Basso-Beattie-
Bresnahan (BBB),
CCombined
Behavior Score
(CBS) test, and
histological
evaluation
Empty
chitosan
tube
2 months
Laminin-coated
chitosan tube
improves functional
recovery by guiding
damaged axon
regrowth, through
the lesioned area,
without inducing
inflammation or
apoptosis
Cheng,
Huang,
Chang,
and
Huang
(2007)
Polyglycolic
acid-
chitosan
tube
Coating with
recombinant
L1-Fc
-
Rats
Optic nerve
transection
-
Anterograde and
retrograde tracing
and histological
evaluation
Polyglycolic
acid-
chitosan
tube
-
The polyglycolic
acid-chitosan
conduit coated with
L1-Fc is more
effective to promote
axonal regeneration
and remyelination
Xu et al.
(2004)
