Biology Reference
In-Depth Information
Follow-up (1-10 min)
Follow-up (1-10 min)
200
200
*
+37%
100
100
0
0
Young
Mature
Young
Mature
Figure 4.5 Comparative effect of 632-nm laser irradiation on CK activity and DNA syn-
thesis in young and mature skeletal muscle cells. To evaluate the laser treatment effect,
we calculated all cells results between 1 and 10 min as there is no trend of change in
these time points. Asterisks denote significant
p
-values (
*
p<
0.05).
and 10 min as there were no trend change in these time points. The laser
effect on CK activity increased in the mature cells in comparison with young
cells (
p
<
0.05).
3. DISCUSSION AND FUTURE PERSPECTIVES
In this study, we have investigated the influence of low-power laser
irradiation on CK activity and the amount AChRs in intact gastrocnemius
muscle (
in vivo
), and on the synthesis of DNA and CK in muscle cells (
in vitro
)
in order to estimate biochemical transformation on cellular and biochemical
levels.
Posttraumatic nerve repair and prevention of muscle atrophy represent a
major challenge of restorative medicine. Among the possible explanations
for the incomplete restoration of very long term denervated muscle are fail-
ures of regenerating nerves to reach all of the atrophic muscle fibers and
establish mature muscle-nerve contacts (
Fu and Gordon, 1995; Irintchev,
Draguhn, & Wernig, 1990
). It was shown earlier that the sites of former
endplates could be detected in surviving skeletal muscle fibers even after
17 months (
Sunderland and Ray, 1950
) and 2 years of muscle denervation
(
Debkov, Kostrominova, Borisov, & Carlson, 2001
). Nevertheless, it is very
difficult to assess the functional condition of those long-term denervated
sites of former neuromuscular junctions with respect to their capacity to
accept growing axons if reinnervation was to occur. Considerable interest
