Biology Reference
In-Depth Information
are associated with cessation of cell replication and elevation of a number of
muscle-specific proteins (
Kloosterboer, Faassen, Stroker-de Vries, &
Hommes, 1979
), including CK and AChR (
Shainberg and Burstein, 1976
).
2. PHOTOTHERAPY INCREASES SKELETAL MUSCLE
BIOCHEMICAL ACTIVITY
In
in vivo study
, the influence of low-power laser irradiation on CK
activity and the level of AChR in intact muscle to estimate biochemical
transformation on cellular and biochemical levels during long-term period
of time were investigated.
The rats underwent laser treatment (He-Ne laser, 35 mW, 30 min)
every day, for 14 days. Low-power laser irradiation was delivered tra-
nscutaneously to the intact gastrocnemius muscle. Under general anesthesia,
the rats were sacrificed and the gastrocnemius muscle (none-treated and
laser-treated) was homogenized on day 7, 14, 21, 30, 60, 120, and 210 in
both groups.
CK activity
was measured by the specific spectrophotometrical method
(
Oliver, 1955; Rosaki, 1967
) using a spectrophotometer at 340 nm and a
Sigma kit at 7, 14, 21, 30, 60, 120, and 210 days in both intact (control)
and laser-irradiated intact muscles.
Internal and membrane inserted AChR
was quantitated by the
125
I-
a
-
bungarotoxin on the same homogenates (
Almon, Andrew, & Appel,
1974; Chin and Almon, 1980
) at different time points 7, 14, 21, 30, 60,
120, and 210 days in both control and laser-irradiated muscles. The data
obtained were evaluated as cpm of bound
125
I-
a
-bungarotoxin/mg protein.
Radioactivity was assessed using Gamma Counter in both muscles.
In an
in vitro
study, the thigh muscles were dissected from 2- to 3-day-old
rats. Single cells were dissociated by trypsin. The cells were preplated into a
plastic culture dish and then plated 1.5
10
6
cells into collagen-coated dis-
hes (35 mm, Nunc) containing 1.5 ml of growth medium. The growth
medium contained Dulbecco's modified Eagle's medium, supplemented
with 10% horse serum and 2% chick embryo extract. The cultures were
maintained at 37
C in an atmosphere of 5% CO
2
, 95% air, in a water sat-
urable incubator. The cultures were then processed for protein and CK
activity. DNA synthesis was investigated on two cell culture models: myo-
blast cultures (young cells) and muscle fibers (mature cells) grown in culture
for 7 days.
