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cells by building several operational in vivo digital logic circuits, each composed
of three gates that have been optimized by genetic process engineering.
Because of the limits to single-cell circuit complexity, and to demonstrate
coordinated behavior in cell aggregates, we also engineered intercellular com-
munications. The mechanism relies on programmed enzymatic activity and
chemical diffusions to carry messages, using DNA from the Vibrio fischeri lux
operon. We built and characterized several circuits that integrate intracellular
logic with lux operon-based intercellular communications.
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