Information Technology Reference
In-Depth Information
Table 7.1 Signaling systems similar to VAI:
N
-acyl-
L
-homoserine lactone
autoinducers in bacteria.
Species
Relation to host Regulates
I gene R gene
Vibrio fischeri
Marine symbiont
Bioluminescence
luxI
luxR
Vibrio harveyi
Marine symbiont
Bioluminescence
luxI
,M
luxN,P,Q
Pseudomonas aeruginosa
Human pathogen
Virulence factors
lasI
lasR
Rhamnolipids
rhlI
rhlR
Yersinia enterocolitica
Human pathogen
?
yenI
yenR
Chromobacterium
violaceum
Human pathogen
Violaceum
production,
hemolysin,
exoprotease
cviI
cviR
Enterobacter
agglomerans
Human pathogen
?
eagI
?
Agrobacterium
tumefaciens
Plant pathogen
Ti plasmid
conjugation
traI
traR
Erwinia caratovora
Plant pathogen
Virulence factors,
carbapenem
expI
expR
Erwinia stewartii
Plant pathogen
Extracellular
capsule
esaI
esaR
Rhizobium
leguminosarum
Plant symbiont
Rhizome
interactions
rhiI
rhiR
Pseudomonas
aureofaciens
Plant beneficial
Phenazine
production
phzI
phzR
one such communication mechanism, realistic genetically controlled develop-
mental systems will require perhaps dozens of such signals. The
LasI
/
LasR
system from
Pseudomonas aeruginosa
[1], for example, appears to encode a
similar regulatory system, but one that uses a different, and non-cross-reacting
autoinducer and a different structure homologous to the Lux box. Table 7.1
lists additional signaling systems similar to VAI that could serve as potential
communication signals [14]. Isolation and characterization of such additional
communication mechanisms will allow the construction of more complex multi-
cellular systems.
CONCLUSIONS
An important element in biocircuit design is genetic process engineering, a
methodology for mutating the DNA encoding of existing genetic elements to
achieve the desired input/output behavior for constructing reliable circuits of
significant complexity. The optimized components we synthesized with this
process exhibit the desired signal restoration and noise margins for reliable
digital computation. We demonstrated the feasibility of digital computation in
